5NQX
Structure of a fHbp(V1.1):PorA(P1.16) chimera. Fusion at fHbp position 294.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97858 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 94.850, 114.900, 160.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.636 - 3.660 |
| R-factor | 0.2348 |
| Rwork | 0.232 |
| R-free | 0.28150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ayd |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.424 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 3.760 |
| High resolution limit [Å] | 3.660 | 3.660 |
| Rmerge | 0.187 | 0.833 |
| Rpim | 0.101 | 0.439 |
| Number of reflections | 19820 | 1431 |
| <I/σ(I)> | 7.6 | 1.9 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 4.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 20% (w/v) PEG4000, 0.3M ammonium sulphate |
