5NQW
IgE-Fc in complex with single domain antibody 026
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96860 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 102.020, 102.020, 300.424 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.993 - 3.400 |
| R-factor | 0.2149 |
| Rwork | 0.212 |
| R-free | 0.24090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wqr |
| RMSD bond length | 0.004 |
| RMSD bond angle | 4.717 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.400 |
| Number of reflections | 22583 |
| <I/σ(I)> | 8.3 |
| Completeness [%] | 99.0 |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M imidazole pH 7.0 11-12 % polyethylene glycol 20.000 |
