5NQI

E.coli 16S rRNA Sarcin-Ricin Loop containing a 5-hydroxymethylcytosine modification

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.77491
Spacegroup name	P 43
Unit cell lengths	29.510, 29.510, 76.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.510 - 0.851
R-factor	0.1737
Rwork	0.146
R-free	0.17040
RMSD bond length	0.006
RMSD bond angle	1.124
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	0.850
Number of reflections	1277131
<I/σ(I)>	30
Completeness [%]	99.6
Redundancy	22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	3.0-3.2 M (NH4)2SO4, 50 mM KMops (pH 7.0), 10 mM MgCl2, 10 mM MnCl2