5NQI
E.coli 16S rRNA Sarcin-Ricin Loop containing a 5-hydroxymethylcytosine modification
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-27 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.77491 |
Spacegroup name | P 43 |
Unit cell lengths | 29.510, 29.510, 76.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.510 - 0.851 |
R-factor | 0.1737 |
Rwork | 0.146 |
R-free | 0.17040 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.124 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 0.850 |
Number of reflections | 1277131 |
<I/σ(I)> | 30 |
Completeness [%] | 99.6 |
Redundancy | 22 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3.0-3.2 M (NH4)2SO4, 50 mM KMops (pH 7.0), 10 mM MgCl2, 10 mM MnCl2 |