5NQH
Structure of the human Fe65-PTB2 homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.578, 104.279, 60.578 |
| Unit cell angles | 90.00, 112.04, 90.00 |
Refinement procedure
| Resolution | 49.440 - 2.600 |
| R-factor | 0.1952 |
| Rwork | 0.193 |
| R-free | 0.24050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dxc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.583 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.440 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 19955 | |
| <I/σ(I)> | 9 | 2.3 |
| Completeness [%] | 99.3 | 98.3 |
| Redundancy | 6.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.08 M sodium acetate, 1.6 M ammonium sulfate, 20 % (v/v) glycerol |
