5NQB
Rabbit Muscle L-lactate dehydrogenase in complex with malonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-14 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.806, 76.952, 118.858 |
| Unit cell angles | 90.00, 96.48, 90.00 |
Refinement procedure
| Resolution | 29.423 - 1.580 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h3f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.423 |
| High resolution limit [Å] | 1.580 |
| Rmerge | 0.069 |
| Number of reflections | 318978 |
| <I/σ(I)> | 8 |
| Completeness [%] | 89.7 |
| Redundancy | 2.1 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | The crystallisation condition is 10% w/v PEG3350 and 0.1 M sodium malonate. |
