5NPZ
Porcine (Sus scrofa) Major Histocompatibility Complex, class I, presenting EFEDLTFLA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.92819 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.034, 128.290, 46.794 |
Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
Resolution | 41.170 - 1.430 |
R-factor | 0.1855 |
Rwork | 0.184 |
R-free | 0.21370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PHASER |
RMSD bond length | 0.019 |
RMSD bond angle | 1.801 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER (2.6.1) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.750 | 42.750 | 1.470 |
High resolution limit [Å] | 1.430 | 6.400 | 1.430 |
Rmerge | 0.058 | 0.021 | 0.881 |
Rmeas | 0.068 | 0.025 | 1.049 |
Rpim | 0.035 | 0.012 | 0.562 |
Number of reflections | 89178 | ||
<I/σ(I)> | 10.7 | ||
Completeness [%] | 99.9 | 99.7 | 99.7 |
Redundancy | 3.6 | 4 | 3.1 |
CC(1/2) | 0.998 | 0.999 | 0.568 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | TOPS screen, condition B02: 20% PEG 4000, 15% Glycerol, 0.1 M Sodium Cacodylate, pH 6.5 |