5NPV
Structure of human ATG5-ATG16L1(ATG5BD) complex (I4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-26 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | I 4 |
Unit cell lengths | 143.580, 143.580, 62.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.404 - 3.100 |
R-factor | 0.2543 |
Rwork | 0.253 |
R-free | 0.27810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gdk |
RMSD bond length | 0.003 |
RMSD bond angle | 0.489 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.404 | 45.404 | 3.200 |
High resolution limit [Å] | 3.100 | 10.000 | 3.100 |
Rmerge | 0.167 | 0.025 | 0.723 |
Rmeas | 0.185 | 0.028 | 0.799 |
Number of reflections | 11680 | 360 | 1069 |
<I/σ(I)> | 11.45 | 49.09 | 2.22 |
Completeness [%] | 99.6 | 94.5 | 100 |
Redundancy | 5.51 | 4.956 | 5.547 |
CC(1/2) | 0.992 | 0.999 | 0.671 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.76 | 293 | 0.1 M MES pH 5.76, 0.378 M KCl, 21.7 % PEG 3350 |