5NPM

CRYSTAL STRUCTURE OF MUTANT RIBOSOMAL PROTEIN TTHL1 LACKING 8 N-TERMINAL RESIDUES IN COMPLEX WITH 80NT 23S RNA FROM THERMUS THERMOPHILUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	110
Detector technology	CCD
Collection date	2011-11-09
Detector	Bruker Platinum 135
Wavelength(s)	1.54179
Spacegroup name	P 21 21 21
Unit cell lengths	64.127, 65.532, 119.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.341 - 2.700
R-factor	0.2042
Rwork	0.202
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u4m
RMSD bond length	0.006
RMSD bond angle	1.037
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Number of reflections	14072
<I/σ(I)>	8.43
Completeness [%]	96.9
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	2.4 M sodium malonate