5NPH
Structure of the Hepatitis C virus strain J4 glycoprotein E2 antigenic region 532-540 bound to the Fab fragment of the non-neutralizing antibody DAO5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98011 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.678, 80.717, 54.565 |
Unit cell angles | 90.00, 95.64, 90.00 |
Refinement procedure
Resolution | 34.000 - 1.700 |
R-factor | 0.18 |
Rwork | 0.179 |
R-free | 0.20250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Fab fragment |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.440 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.040 | 0.228 |
Number of reflections | 44810 | 6417 |
<I/σ(I)> | 27.6 | 6.8 |
Completeness [%] | 97.1 | 95.6 |
Redundancy | 5.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG3350, 200mM sodium thiocyanate |