5NOG
Ca2+-induced Movement of Tropomyosin on Native Cardiac Thin Filaments - "Blocked" state
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 11 |
| Software | SPIDER,CTFFIND,DIREX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
