5NN8

Crystal structure of human lysosomal acid-alpha-glucosidase, GAA, in complex with acarbose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.872600
Spacegroup name	P 21 21 21
Unit cell lengths	97.050, 102.986, 127.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.550 - 2.450
R-factor	0.17611
Rwork	0.174
R-free	0.21231
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5nn3
RMSD bond length	0.007
RMSD bond angle	1.344
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.550	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.188	0.885
Rpim	0.116	0.554
Number of reflections	44773	4760
<I/σ(I)>	8	1.9
Completeness [%]	94.7	97
Redundancy	5.3	5.2
CC(1/2)	0.986	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.9 M ammonium sulphate, 0.1 M HEPES pH 7.0, 2% v/v PEG400