5NN3
Crystal structure of human lysosomal acid-alpha-glucosidase, GAA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.660, 102.401, 128.751 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.880 - 1.900 |
| R-factor | 0.16328 |
| Rwork | 0.162 |
| R-free | 0.18874 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qly |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.404 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.103 | 1.711 |
| Rpim | 0.050 | 0.823 |
| Number of reflections | 102224 | 5033 |
| <I/σ(I)> | 13.7 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9 | 9.2 |
| CC(1/2) | 0.992 | 0.661 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.9 M ammonium sulfate, 0.1 M HEPES pH 7.0, 2% v/v PEG400 |
