5NL7
The crystal structure of the Actin Binding Domain (ABD) of alpha actinin from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.84260 |
| Spacegroup name | P 3 |
| Unit cell lengths | 146.740, 146.740, 33.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.032 - 2.480 |
| R-factor | 0.1991 |
| Rwork | 0.196 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wku |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.956 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.032 | 2.540 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.101 | 0.647 |
| Number of reflections | 27759 | 1951 |
| <I/σ(I)> | 8.33 | 2.03 |
| Completeness [%] | 95.6 | 90.5 |
| Redundancy | 3.1 | |
| CC(1/2) | 0.995 | 0.398 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.2 M Ca(CH3COO)2, 0.1 M Sodium Cacodylate pH 6.5 and 40% v/v PEG600 |
