5NKJ
X-ray structure of the N239C mutant of GLIC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-03-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.140, 134.690, 158.620 |
| Unit cell angles | 90.00, 99.99, 90.00 |
Refinement procedure
| Resolution | 14.000 - 3.740 |
| R-factor | 0.2347 |
| Rwork | 0.234 |
| R-free | 0.25570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.210 | 4.100 |
| High resolution limit [Å] | 3.200 | 3.900 |
| Rmerge | 0.121 | 0.750 |
| Number of reflections | 58339 | |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 98.5 | 99.8 |
| Redundancy | 3.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | PH 4, VAPOR DIFFUSION, TEMPERATURE 293K |
