5NKG
Crystal Structure of Ephrin A2 (EphA2) Receptor Protein Kinase with Compound 3d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.736, 107.202, 40.471 |
| Unit cell angles | 90.00, 108.75, 90.00 |
Refinement procedure
| Resolution | 29.110 - 1.100 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i9u |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.519 |
| Data reduction software | XDS (XDSapp 2.0) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.106 | 1.170 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmeas | 0.060 | 0.696 |
| Number of reflections | 100653 | 6951 |
| <I/σ(I)> | 12.05 | 1.75 |
| Completeness [%] | 94.1 | 91.4 |
| Redundancy | 2.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 37.5% MPD/PEG1000/PEG3350 (MD), 0.1 M Amino Acids Mix (MD), 0.1 M Tris pH6.5 |
