5NIS
Neutral trehalase Nth1 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-15 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8729 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 187.308, 187.308, 119.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.296 - 3.155 |
| R-factor | 0.2007 |
| Rwork | 0.198 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jta |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.151 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 3.268 |
| High resolution limit [Å] | 3.155 | 3.155 |
| Rmerge | 0.328 | 1.698 |
| Number of reflections | 21792 | 2110 |
| <I/σ(I)> | 11.69 | |
| Completeness [%] | 99.5 | 97.91 |
| Redundancy | 21.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 291 | 100 mM sodium citrate, 1 M lithium sulphate, and 400 mM ammonium sulphate |
