5NIM
EthR complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9600 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 122.544, 122.544, 33.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.650 - 1.500 |
| R-factor | 0.1766 |
| Rwork | 0.175 |
| R-free | 0.20424 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.698 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.650 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.570 |
| Number of reflections | 41904 | 13798 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | MES buffer, 1.4-1.6 M Ammonium sulfate |
