5NH1

Structure of the C-terminal domain of human Gasdermin D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999998
Spacegroup name	P 21 21 2
Unit cell lengths	125.730, 47.980, 37.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.827 - 2.040
R-factor	0.2328
Rwork	0.231
R-free	0.26370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b5r
RMSD bond length	0.002
RMSD bond angle	0.553
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2131: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.827	2.160
High resolution limit [Å]	2.040	2.040
Rmerge	0.196	0.956
Number of reflections	14421	2240
<I/σ(I)>	8.63	2.2
Completeness [%]	95.0	96.1
Redundancy	8.4	8.6
CC(1/2)	0.992	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		303	1.229 M NaCitrate, HEPES, pH 7.25