5NGZ
Ube2T in complex with fragment EM04
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.817, 55.817, 185.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.440 - 2.400 |
| R-factor | 0.21192 |
| Rwork | 0.210 |
| R-free | 0.24734 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yh2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.312 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.450 | |
| High resolution limit [Å] | 2.400 | |
| Rmerge | 0.888 | |
| Number of reflections | 12037 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 99.2 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | potassium acetate, Tris-HCl pH 8.5 |
