5NGD
Crystal structure of the V325G mutant of the bacteriophage G20C portal protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97883 |
| Spacegroup name | H 3 |
| Unit cell lengths | 223.234, 223.234, 115.817 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 111.620 - 1.900 |
| R-factor | 0.20623 |
| Rwork | 0.206 |
| R-free | 0.22512 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.724 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.620 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.168 | 0.736 |
| Number of reflections | 165587 | 8194 |
| <I/σ(I)> | 3.3 | 1.1 |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 2.1 | 2.1 |
| CC(1/2) | 0.943 | 0.401 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Protein in 1 M NaCl, 20 mM Tris pH 7.5. Reservoir: 0.1 M Imidazole HCl pH 8.0, 30% (w/v) MPD, 10% (w/v) PEG 4000. |
