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5NGD

Crystal structure of the V325G mutant of the bacteriophage G20C portal protein

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I24
Synchrotron siteDiamond
BeamlineI24
Temperature [K]100
Detector technologyPIXEL
Collection date2016-09-24
DetectorDECTRIS PILATUS3 6M
Wavelength(s)0.97883
Spacegroup nameH 3
Unit cell lengths223.234, 223.234, 115.817
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution111.620 - 1.900
R-factor0.20623
Rwork0.206
R-free0.22512
Structure solution methodSAD
RMSD bond length0.017
RMSD bond angle1.724
Data reduction softwareDIALS
Data scaling softwareAimless
Phasing softwareSHELX
Refinement softwareREFMAC (5.8.0158)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]111.6201.930
High resolution limit [Å]1.9001.900
Rmerge0.1680.736
Number of reflections1655878194
<I/σ(I)>3.31.1
Completeness [%]97.897.8
Redundancy2.12.1
CC(1/2)0.9430.401
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8293Protein in 1 M NaCl, 20 mM Tris pH 7.5. Reservoir: 0.1 M Imidazole HCl pH 8.0, 30% (w/v) MPD, 10% (w/v) PEG 4000.

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