5NGD
Crystal structure of the V325G mutant of the bacteriophage G20C portal protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97883 |
Spacegroup name | H 3 |
Unit cell lengths | 223.234, 223.234, 115.817 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 111.620 - 1.900 |
R-factor | 0.20623 |
Rwork | 0.206 |
R-free | 0.22512 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.724 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.620 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.168 | 0.736 |
Number of reflections | 165587 | 8194 |
<I/σ(I)> | 3.3 | 1.1 |
Completeness [%] | 97.8 | 97.8 |
Redundancy | 2.1 | 2.1 |
CC(1/2) | 0.943 | 0.401 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Protein in 1 M NaCl, 20 mM Tris pH 7.5. Reservoir: 0.1 M Imidazole HCl pH 8.0, 30% (w/v) MPD, 10% (w/v) PEG 4000. |