5NFZ

TUBULIN-MTC complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97976
Spacegroup name	P 21 21 21
Unit cell lengths	105.163, 158.787, 180.376
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.782 - 2.100
R-factor	0.1839
Rwork	0.182
R-free	0.21760
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4i4t
RMSD bond length	0.007
RMSD bond angle	0.856
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.782	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.135	2.890
Rpim	0.042	0.879
Number of reflections	175862	12854
<I/σ(I)>	16.2	0.92
Completeness [%]	100.0	99.8
Redundancy	13.5	10.6
CC(1/2)	0.999	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	5% PEG 4K, 12-14% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole, pH 6.7