5NF6
Structure of GluK3 ligand-binding domain (S1S2) in complex with CIP-AS at 2.55 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 130.138, 56.071, 87.516 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.480 - 2.550 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u92 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.528 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.210 | 2.690 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.484 | |
Number of reflections | 21596 | |
<I/σ(I)> | 20 | 1.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG8000, 5 mM Zinc Acetate, 0.1 M Tris pH 8.0 |