5NEZ

crystal structure of variants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-16
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	76.978, 136.409, 152.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.409 - 2.394
R-factor	0.1786
Rwork	0.176
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.973
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2119: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.409
High resolution limit [Å]	2.394
Rmerge	0.079	0.518
Rmeas	0.082	0.539
Rpim	0.022	0.147
Number of reflections	63701
<I/σ(I)>	26.06
Completeness [%]	99.5
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	PEG, Tris cl