5NDI
The structure of the E.coli guanidine II riboswitch P1 stem-loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.476, 48.185, 153.066 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.344 - 2.570 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.244 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.020 | 2.610 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.082 | 1.460 |
| Rpim | 0.038 | 0.690 |
| Number of reflections | 20792 | |
| <I/σ(I)> | 10.7 | 1.1 |
| Completeness [%] | 100.0 | 99.3 |
| Redundancy | 5.38 | 5.3 |
| CC(1/2) | 0.998 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.01 M Magnesium Chloride, 0.05 M MES pH 5.6, 2.0 M Lithium Sulfate monohydrate |
