5NCL
Crystal structure of the Cbk1-Mob2 kinase-coactivator complex with an SSD1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.430, 79.990, 117.590 |
| Unit cell angles | 90.00, 117.60, 90.00 |
Refinement procedure
| Resolution | 44.595 - 3.150 |
| R-factor | 0.2367 |
| Rwork | 0.233 |
| R-free | 0.29820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5brk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.292 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2420: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.595 | 3.230 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.168 | 1.040 |
| Number of reflections | 19888 | 1455 |
| <I/σ(I)> | 9.37 | 2.05 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 7.7 | 7.8 |
| CC(1/2) | 0.996 | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 296 | 25% PEG 20,000 BUFFERED WITH 0.1M NA-CITRATE |
