5NBQ

The structure of the tripartite complex between OspE, the C-terminal domains of factor H and C3dg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.969
Spacegroup name	P 21 21 2
Unit cell lengths	124.907, 165.063, 84.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 3.180
R-factor	0.235
Rwork	0.233
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.600	29.600	3.370
High resolution limit [Å]	3.180	9.130	3.180
Rmerge	0.135	0.033	0.892
Rmeas	0.147	0.036	0.983
Total number of observations	186599
Number of reflections	29070	1346	3897
<I/σ(I)>	12.43	44.89	1.73
Completeness [%]	96.9	96	82.3
Redundancy	6.419	5.808	5.399
CC(1/2)	0.997	0.999	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or 24% PEG3350, 0.1 M HEPES pH 7.5, 0.2 M MgCl2 or 16% PEG3350, Tris pH 8.5, 0.2 M ammonium acetate