5NBD
PglK flippase in complex with inhibitory nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 84.340, 142.660, 199.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.349 - 3.900 |
| R-factor | 0.313 |
| Rwork | 0.311 |
| R-free | 0.33500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5C78 and 4UU9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.607 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.349 | 4.000 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmeas | 0.107 | |
| Number of reflections | 42284 | |
| <I/σ(I)> | 13 | 1.02 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.3 | 298 | MOPS: NaCl: PEG300 |
