5N8L
Structure of TRBP dsRBD 1 and 2 in complex with a 19 bp siRNA (Complex B)
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE III | 750 | |
| 2 | Bruker | AVANCE III | 900 | |
| 3 | Bruker | AVANCE III | 600 | |
| 4 | Bruker | AVANCE III | 500 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | hCccoNH |
| 10 | 1 | 3 | 3D f1-13C-filtered,f2-13C-edited NOESY aliphatic-HMQC |
| 11 | 1 | 4 | TROSY IPAP |
| 12 | 1 | 5 | TROSY IPAP |
| 2 | 1 | 1 | 3D 1H-13C NOESY aliphatic |
| 3 | 1 | 1 | 3D 1H-15N NOESY |
| 4 | 1 | 1 | 3D HNCACB |
| 5 | 1 | 1 | 3D CBCA(CO)NH |
| 6 | 1 | 2 | 3D HCCH-TOCSY |
| 7 | 1 | 2 | 3D 1H-13C NOESY aliphatic |
| 8 | 1 | 2 | 3D 1H-15N NOESY |
| 9 | 1 | 2 | 3D HNCA |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.5 | AMBIENT | 313 |
Conformers
| Conformers Calculated Total Number | 30 |
| Conformers Submitted Total Number | 20 |
