5N8A

Structure of RPA70N in complex with PrimPol (fragment 480-560)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91407
Spacegroup name	P 21 21 21
Unit cell lengths	38.050, 53.490, 53.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.247 - 1.280
R-factor	0.1549
Rwork	0.154
R-free	0.17850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ipc
RMSD bond length	0.007
RMSD bond angle	0.912
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	16.247	16.247	1.310
High resolution limit [Å]	1.280	5.720	1.280
Rmerge	0.043	0.019	0.604
Rmeas	0.047	0.020	0.700
Total number of observations	351864
Number of reflections	351864	595	4028
<I/σ(I)>	20.01	73.95	1.87
Completeness [%]	99.7	95	97.7
Redundancy	6.431	6.931	3.845
CC(1/2)	1.000	1.000	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293.15	0.2M imidazole malate, 30% w/v PEG 4000