5N86

Crystal structure of FAS1 domain of hyaluronic acid receptor stabilin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	48.740, 55.830, 87.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.069 - 1.484
R-factor	0.1464
Rwork	0.145
R-free	0.17170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o70
RMSD bond length	0.008
RMSD bond angle	1.013
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.069	48.740	1.560
High resolution limit [Å]	1.484	4.690	1.484
Rmerge		0.068	0.183
Rmeas	0.094	0.075	0.201
Rpim	0.038	0.031	0.082
Number of reflections	40147
<I/σ(I)>	10.9	7.9	3.2
Completeness [%]	99.8	99.7	100
Redundancy	5.7	5.4	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.1 M Tris pH 8.5, 0.8 M sodium/potassium tartrate, 0.5% PEG 5000 MME