5N77
Crystal structure of the cytosolic domain of the CorA magnesium channel from Escherichia coli in complex with magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.313, 117.453, 91.114 |
| Unit cell angles | 90.00, 103.27, 90.00 |
Refinement procedure
| Resolution | 29.840 - 2.800 |
| R-factor | 0.19672 |
| Rwork | 0.195 |
| R-free | 0.23591 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.371 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.840 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.129 | 0.836 |
| Number of reflections | 36878 | |
| <I/σ(I)> | 13 | 2.4 |
| Completeness [%] | 99.0 | 93.5 |
| Redundancy | 6.2 | 5.8 |
| CC(1/2) | 0.995 | 0.746 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20 mM MgCl2; 100 mM Glycine pH8.7; 8-18% PEG400; 0,5-2% TritonX-114. |
