5N6L

Structure of the membrane integral lipoprotein N-acyltransferase Lnt C387A mutant from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99998
Spacegroup name	P 21 21 21
Unit cell lengths	55.220, 142.950, 197.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.790 - 2.900
R-factor	0.2288
Rwork	0.227
R-free	0.26330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n6h
RMSD bond length	0.004
RMSD bond angle	0.798
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER

Data quality characteristics

	Overall
Low resolution limit [Å]	49.790
High resolution limit [Å]	2.900
Number of reflections	34916
<I/σ(I)>	7.72
Completeness [%]	97.9
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	8 % (v/v) MPD 0.1 M MES pH 6 0.4 M ammonium citrate 0.1 M sodium malonate pH 7.0