5N6H

Structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99997
Spacegroup name	P 21 21 21
Unit cell lengths	54.200, 142.300, 199.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.099 - 2.900
R-factor	0.2204
Rwork	0.219
R-free	0.24940
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.728
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	BUSTER

Data quality characteristics

	Overall
Low resolution limit [Å]	49.100
High resolution limit [Å]	2.900
Number of reflections	693957
<I/σ(I)>	9.5
Completeness [%]	100.0
Redundancy	19.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	8 % (v/v) MPD 0.1 MES pH 6.0 0.4 M ammonium citrate