5N26
X-ray structure of human heavy chain ferritin in complex with cisplatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-15 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.069, 183.069, 183.069 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.690 - 2.050 |
| R-factor | 0.1646 |
| Rwork | 0.163 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y08 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.919 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.700 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.143 | 0.588 |
| Rpim | 0.053 | 0.236 |
| Number of reflections | 16819 | |
| <I/σ(I)> | 17.8 | 3.7 |
| Completeness [%] | 98.5 | 91.8 |
| Redundancy | 7.9 | 6.9 |
| CC(1/2) | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 2.0 M Magnesium chloride, 0.1 M bicine pH 9.0 |
