5N0B
Crystal structure of the tetanus neurotoxin in complex with GD1a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9282 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 152.695, 136.840, 92.630 |
Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.500 | 2.860 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.134 | 0.937 |
Rpim | 0.088 | 0.644 |
Number of reflections | 58411 | 27187 |
<I/σ(I)> | 10 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.3 | 6 |
CC(1/2) | 0.996 | 0.307 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% v/v polyethylene glycol 3350, 0.1M Bis-Tris propane pH 6.5, 0.2M potassium thiocyanate |