5N0A
Crystal structure of A259C covalently linked dengue 2 virus envelope glycoprotein dimer in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 A11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2015-03-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 58.774, 182.301, 208.725 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.900 |
| R-factor | 0.2861 |
| Rwork | 0.285 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4UTA;4UT7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.100 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.316 | 0.800 |
| Rmeas | 0.410 | |
| Rpim | 0.257 | 0.670 |
| Number of reflections | 19112 | 4789 |
| <I/σ(I)> | 3.1 | 1.4 |
| Completeness [%] | 96.9 | 97.5 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100mM Hepes pH 7.5, 19% PEG 6K, 1.5% (v/v) 2-methyl-2,4-pentanediol |
