5N06
Crystal structure of Tie1 Fibronectin-like domain 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 65 |
| Unit cell lengths | 54.054, 54.054, 107.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.416 - 2.501 |
| R-factor | 0.2489 |
| Rwork | 0.243 |
| R-free | 0.31510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Tie2 Fn3 domain |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.787 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 6161 | 951 |
| <I/σ(I)> | 15.2 | 2 |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 7.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Tris buffer at pH 8.0 - 9.0 and 18-24% PEG 8000 (w/v) |
