5MYB

Homodimerization of Tie2 Fibronectin-like domains 2 and 3 in space group P21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	78.460, 48.490, 80.670
Unit cell angles	90.00, 111.09, 90.00

Refinement procedure

Resolution	39.800 - 2.600
R-factor	0.225
Rwork	0.222
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CRYSTAL STRUCTURE OF TIE2 FN-LIKE DOMAINS IN SPACE GROUP C2
RMSD bond length	0.007
RMSD bond angle	1.271
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Outer shell
Low resolution limit [Å]	2.760
High resolution limit [Å]	2.600
<I/σ(I)>	1.8
Completeness [%]	93.6
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	294	0.1 M Tris buffer at pH 7.0 - 8.5 and 14-20% PEG 3350 (w/v)