5MYA
Homodimerization of Tie2 Fibronectin-like domains 1-3 in space group C2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-30 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9677 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 134.160, 104.690, 79.520 |
Unit cell angles | 90.00, 121.41, 90.00 |
Refinement procedure
Resolution | 29.419 - 2.900 |
R-factor | 0.2368 |
Rwork | 0.234 |
R-free | 0.27720 |
Structure solution method | SIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.142 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 20882 | 3444 |
<I/σ(I)> | 16.1 | 1.5 |
Completeness [%] | 99.4 | 99.5 |
Redundancy | 11.4 | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Tris buffer at pH 7.0 - 8.5 and 14-20% PEG 3350 (w/v) |