5MXM
The X-ray structure of human M190I phosphoglycerate kinase 1 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-23 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.894 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.363, 91.104, 109.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.910 - 2.050 |
| R-factor | 0.17861 |
| Rwork | 0.176 |
| R-free | 0.22554 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.953 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 69.910 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 24685 |
| <I/σ(I)> | 13.15 |
| Completeness [%] | 99.7 |
| Redundancy | 5.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG MME 30% w/v, Bis Tris 0.1 M pH=7 |
