5MX0

Crystal structure of human fibromodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9282
Spacegroup name	C 1 2 1
Unit cell lengths	108.110, 98.930, 111.340
Unit cell angles	90.00, 107.39, 90.00

Refinement procedure

Resolution	65.913 - 2.210
R-factor	0.1812
Rwork	0.179
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xku
RMSD bond length	0.003
RMSD bond angle	0.759
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.913	2.270
High resolution limit [Å]	2.210	2.210
Rmerge	0.111	1.840
Rpim	0.046	0.737
Number of reflections	55434
<I/σ(I)>	9.5	1.1
Completeness [%]	99.0	99.1
Redundancy	6.8	6.9
CC(1/2)	0.998	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	10 mg/ml protein + 26% (w/v) PEG 3350, 200 mM lithium sulphate monohydrate, 100 mM Tris-HCl (pH 8.5)