5MTO

N-terminal domain of the human tumor suppressor ING5 C19S mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98011
Spacegroup name	P 65 2 2
Unit cell lengths	140.940, 140.940, 92.323
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	121.000 - 3.100
R-factor	0.2173
Rwork	0.215
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5me8
RMSD bond length	0.007
RMSD bond angle	1.024
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	122.058	73.632	3.270
High resolution limit [Å]	3.100	9.800	3.100
Rmerge		0.094	0.938
Rmeas	0.179	0.098	0.964
Rpim	0.042	0.025	0.221
Number of reflections	10298
<I/σ(I)>	9.7	2.9	0.8
Completeness [%]	100.0	99.9	100
Redundancy	18.3	15.7	18.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.78 M NPS 0.08 HEPES/MOPS Buffer pH 7.5 32.8% MPD_PEG1K_PEG3350