5MTC
Crystal structure of PDF from the Vibrio parahaemolyticus bacteriophage VP16T - crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979783 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.730, 64.730, 128.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.260 - 1.700 |
| R-factor | 0.17219 |
| Rwork | 0.171 |
| R-free | 0.20135 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lry |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.296 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 35064 | 5554 |
| <I/σ(I)> | 20.33 | 3.28 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 8.97 | 8.93 |
| CC(1/2) | 0.999 | 0.889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 292 | 38% MPD 100mM CAPS |
