5MT0
COMPLEMENT FACTOR D IN COMPLEX WITH A REVERSIBLE INDOLE CARBOXYLIC ACID BASED INHIBITOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.477, 49.527, 39.263 |
| Unit cell angles | 90.00, 106.36, 90.00 |
Refinement procedure
| Resolution | 29.060 - 1.290 |
| Rwork | 0.154 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1TACE |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.220 | 1.340 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.045 | 0.326 |
| Number of reflections | 50955 | |
| <I/σ(I)> | 14.62 | 3.7 |
| Completeness [%] | 98.9 | 97.6 |
| Redundancy | 3.28 | 3.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG3350, 100 mM BIS-TRIS pH 6.5 |
