5MRW
Structure of the KdpFABC complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.03666 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 124.720, 166.290, 196.300 |
| Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.2446 |
| Rwork | 0.243 |
| R-free | 0.27490 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.448 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 19.974 | 3.000 |
| High resolution limit [Å] | 2.900 | 15.000 | 2.900 |
| Rmerge | 0.201 | 0.037 | 4.910 |
| Number of reflections | 167947 | ||
| <I/σ(I)> | 11.28 | 52.3 | 0.96 |
| Completeness [%] | 99.2 | 96.1 | 98.9 |
| Redundancy | 18.8 | 18.135 | 18.5 |
| CC(1/2) | 0.999 | 0.999 | 0.488 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 20% (w/v) PEG3350, 0.5-1.0 M NaCl, 0.05 mM sodium citrate pH 5.5. |
