5MQ4
Crystal Structure of the leucine zipper of human PRKCBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.638, 237.049, 210.144 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.625 - 2.700 |
| R-factor | 0.2096 |
| Rwork | 0.208 |
| R-free | 0.23620 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.106 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.150 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 1.143 | |
| Number of reflections | 46326 | |
| <I/σ(I)> | 13.6 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 274 | 1M ammonium sulfate -- 0.1M MES pH 6.3 |
