5MPK
Crystal structure of CREBBP bromodomain complexed with DK19
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.077, 60.204, 87.636 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.811 - 1.900 |
R-factor | 0.1883 |
Rwork | 0.183 |
R-free | 0.23910 |
RMSD bond length | 0.011 |
RMSD bond angle | 0.955 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.080 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.091 |
Number of reflections | 19464 |
<I/σ(I)> | 12 |
Completeness [%] | 99.9 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M HEPES-Na, pH7.5, 0.2 M MgCl2, 25% P3350 |