5MP7
Crystal structure of phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.292, 85.292, 249.791 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.400 |
| R-factor | 0.1996 |
| Rwork | 0.198 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dkr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.650 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.380 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.067 | 0.340 |
| Number of reflections | 35723 | |
| <I/σ(I)> | 6.24 | 2.34 |
| Completeness [%] | 96.2 | 97.26 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277.15 | 0.2M NaCl, 0.1M Sodium acetate, 30% (v/v) 2-Methyl-2,4-pentanediol |
