5MP1
Crystal structure of DC8E8 Fab in the complex with a 14-mer tau peptide at pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 60.370, 82.370, 89.800 |
Unit cell angles | 88.12, 84.78, 89.93 |
Refinement procedure
Resolution | 39.830 - 3.100 |
R-factor | 0.2245 |
Rwork | 0.222 |
R-free | 0.26840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 4OZ4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.432 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.830 | 39.830 | 3.180 |
High resolution limit [Å] | 3.100 | 13.860 | 3.100 |
Rmerge | 0.081 | 0.026 | 0.313 |
Number of reflections | 24488 | ||
<I/σ(I)> | 9.64 | 26.93 | 2.67 |
Completeness [%] | 78.4 | 49.3 | 60.2 |
Redundancy | 1.722 | 1.908 | 1.571 |
CC(1/2) | 0.987 | 0.998 | 0.741 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 20% PEG 4000; 10% 2-Propanol, 0.1 M Na-HEPES |