5MOK
Crystal structure of human IgE-Fc epsilon 3-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.478, 100.357, 77.856 |
| Unit cell angles | 90.00, 97.35, 90.00 |
Refinement procedure
| Resolution | 77.217 - 2.000 |
| R-factor | 0.2036 |
| Rwork | 0.202 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ha0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.619 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.360 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 68470 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.3 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 25% (v/v) PEG1500 0.1M SPG (succinic acid, sodium dihydrogen phosphate and glycine in the ratio 2:7:7) at pH 6.0 |
